Host species-specific metabolic fingerprint database for enterococci and Escherichia coli and its application to identify sources of fecal contamination in surface waters.

Specific Metabolic Fingerprint Database for Enterococci and Escherichia coli and Its Application To Identify Sources of Fecal Contamination in Surface

Total Coliform, E. Coli, and Enterococci Bacteria in Grazed and Wooded Watersheds of the Southern Piedmont

Contamination of surface waters with fecal bacteria from grazinglands is a component of nonpoint source agricultural pollution. Methods are needed to limit the movement of fecal bacteria from grazinglands into surface water. We used two experimental watersheds to test for impact of cattle on total coliform, E. coli, and enterococci bacteria numbers. Grazing cattle elevated these microbe numbers...

Detection of virulence genes in Escherichia coli of an existing metabolic fingerprint database to predict the sources of pathogenic E. coli in surface waters.

A collection of 366 Escherichia coli strains from 10 host groups and surface waters were tested for the presence of 15 virulence genes associated with strains causing intestinal and extra-intestinal infections. The virulence genes included eaeA, VT1, 2 and 2e, LT1, ST1 and 2, Einv gene, EAgg gene, CNF1 and 2, papC, O111 and O157 side chain LPS. Of the 262 strains obtained from nine different ho...

Sources and Sinks of Escherichia coli in Benthic and Pelagic Fish

Escherichia coli and fecal coliform bacteria were isolated from five benthic and four pelagic fish species to determine their role in the fecal contamination of recreational waters. All fish were collected during fall 2006 from Southworth Marsh in the Duluth-Superior Harbor, a public beach that is commonly posted to minimize water contact due to high E. coli levels. Although fecal coliform bact...

Putative temporal variability of Escherichia coli ribotypes from yearling steers.

Escherichia coli is a ubiquitous component of the intestinal microflora of warm-blooded animals, and is an indicator of fecal contamination of surface waters. Ribotype profiling of E. coli is one of several genotypic methods that has been developed to determine the host origin of fecal bacteria. Like most genotypic methods of source tracking, ribotyping requires a host origin database to identi...